Fluorescent Microscope

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A Fluorescent Microscope is an optical microscope useful in examining properties of organic or inorganic substances utilizing the phenomena of fluorescence and phosphorescence. Such type of microscope uses fluorescence to generate an image. It follows the fluorescence microscopy principal by which a sample to be examined is illuminated with light of a wavelength which causes fluorescence in the sample. The emitting light caused by such phenomenon is then detected through a microscope objective.

Two filters are normally used in this technique. One is the illumination (or excitation) filter which ensures the illumination is near monochromatic and at the correct wavelength. The second is the emission (or detection) filter which ascertains that the illumination light source reaches the detector.