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HPLC stands for High-performance Liquid Chromatography. Also referred to as High-pressure Liquid Chromatography sometimes, HPLC is a chromatographic technique that helps in separating a mixture of compounds. The method is principally applied in biochemistry and analytical chemistry to identify, quantify and purify the individual components of mixtures.

HPLC is basically a highly developed form of column chromatography. Unlike other forms of column chromatography in which solvent is being allowed to drip through a column under gravity, it is forced through under high pressures – up to 400 atmospheric pressure – which make it considerably faster. The system also allows a researcher to use much smaller particle size for the column packing material, which provides a bigger surface area for interactions between the stationary phase and the molecules flowing past it.